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KMID : 1161519980020030383
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Animal Cells and Systems
1998 Volume.2 No. 3 p.383 ~ p.388
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Identification and characterization of a RecA-like protein induced by DNA damaging agents in fluorescent pseudomonas sp.
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Kim Ok-Bong
Lim Chae-Kwang
Kim Si-Wouk
Park Jong-Kun
Yoon Seong-Myeong
Lee Jung-Sup
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Abstract
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A RecA?like protein (RecAps) was identified from fluorescent Pseudomonas sp. and the inducible nature of the protein was characterized in detail. It was shown by dose?response and time?course experiments using two DNA damaging agents, nalidixic acid and mitomycin?C, that the cellular level of RecAps protein was increased 3?8 fold compared to that of the control. The most effective doses of nalidixic acid and mitomycin?C for the protein induction were 30 μg/ml and 0.3 μg/ml at the treatment time point of 150 min, respectively. The enhanced level of RecAps protein was gradually decreased to the control level after 10 hr in normal medium. Interestingly, the cellular level of RecAps protein was increased by the same DNA damaging agents even when cell growth was completely inhibited by treatment with 170 μg/ml of chloramphenicol, an inhibitor of protein synthesis, suggesting that new protein synthesis is not required for the induction of RecAps. All these results suggest that a typical SOS repair function driven by RecA?like protein is conserved in Pseudomonas sp. cells as in E. coli.
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KEYWORD
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Pseudomonas sp., RecA-like protein, DNA damage, SOS DNA repair
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